Araldite as an Embedding Medium for Electron Microscopy
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Araldite as an Embedding Medium for Electron Microscopy
Epoxy resins are suitable media for embedding for electron microscopy, as they set uniformly with virtually no shrinkage. A mixture of araldite epoxy resins has been developed which is soluble in ethanol, and which yields a block of the required hardness for thin sectioning. The critical modifications to the conventional mixtures are the choice of a plasticized resin in conjunction with an alip...
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A new water-soluble embedding agent, tr ial p roduc t CIBA X 133/20971 has recently been in t roduced for selected electron microscopic investigations (1). D u r c u p a n subsequently proved to be miscible wi th the water-insoluble epoxy resin Araldi te 2 which is commonly used for tissue embedding (2). In this way, an imal tissues can be embedded in Araldi te wi thout being b rough t into con...
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During an investigation of the effect of ischemia on striated muscle as seen with the electron microscope it became evident that all degrees of disorientation and destruction of tissues were taking place, and it seemed likely that much of the damage occurred during the embedding process. Such damage made it difficult to proceed with experiments in which comparison of normal tissue with any path...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1958
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.4.2.191